Scaffold architecture and cell–matrix mapping

ScaffoldDynamics

Explore scaffold structure, temporal remodeling and cell–matrix interactions through multimodal microscopy.

Detailed specifications
PlatformProprietary laser-scanning nonlinear microscopy
Imaging modesHigh-resolution label-free structural mapping and multi-channel fluorescence analysis
Structural analysisMorphology, fiber organization, porosity and structural integrity of decellularized 3D-bioprinted scaffolds
Dynamic analysisTemporal scaffold evolution, collagen-fiber orientation and remodeling
Living constructsCell distribution, viability and matrix interactions; structural integrity under dynamic conditions
Collagen imagingLabel-free second-harmonic generation (SHG)
3D mappingZ-stacks for cell distribution and penetration depth
Study parametersImaging volume, resolution, time points, sample preparation and quantitative deliverables are selected for the project; universal values are not given in the catalogue

Methods, deliverables & evidence

  • Catalogue illustrates fluorescence-labeled HUVECs/MSCs alongside label-free SHG collagen imaging
  • Z-stacks reveal cell distribution and infiltration depth
  • Reported comparisons at 3 and 10 days illustrate structural remodeling; composite collagen images integrate polarization states and z-planes

Technical catalogue

Specifications summarized from the October 2026 tissue engineering catalogue, printed page 24. Confirm the selected configuration, compatibility and experimental conditions with MAKBY.